Composite

Part:BBa_K3755046:Design

Designed by: Qi Xin   Group: iGEM21_ShanghaiTech_China   (2021-10-13)


Double Regulated(trp+lac) Kill Switch


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1406
    Illegal XbaI site found at 1903
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1406
    Illegal XbaI site found at 1903
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1406
    Illegal XbaI site found at 1903
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1670


Design Notes

The double regulated kill switch is designed to control the life and death of the bacteria more precisely, which can eliminate the influence of leaked expression.


Source

The hok and sok genes are isolated from R1 plasmid of E.coli, and the regulation part of this system is designed by our team members.

References

[1]Kawano M. Divergently overlapping cis-encoded antisense RNA regulating toxin-antitoxin systems from E. coli: hok/sok, ldr/rdl, symE/symR. RNA Biol. 2012 Dec;9(12):1520-7. doi: 10.4161/rna.22757. Epub 2012 Nov 6. PMID: 23131729.